ISSN 0886-3067

Volume No 35 Issue No 4

Etiology and Antimicrobial Susceptibility Pattern of Bacterial Keratitis at a Tertiary Care Hospital

Purpose: To find out the etiology and antimicrobial susceptibility pattern of bacterial keratitis at a tertiary care hospital in Lahore. Study Design: Descriptive cross sectional study. Place and Duration of Study: Postgraduate Medical Institute/Ameer ud Din Medical College/Lahore General Hospital, Lahore, from 2015 – 2018. Material and Methods: Clinically suspected patients of bacterial keratitis were selected from out patient department of Lahore General hospital and corneal scrapings were collected before start of antimicrobial therapy. Patients who were already on antimicrobial therapy and patients with suspected keratitis caused by micro-organism other than bacteria were excluded from the study. The corneal scrapings taken by the ophthalmologist were immediately inoculated on different culture plates with the help of sterilized wire loop in the operation theater. These plates were taken to the laboratory for incubation and further microbiological processing. The scrapings from cornea were also smeared on glass slides with the help of sterilized wire loop for staining. The culture and staining were further processed for microbiological analysis of bacterial etiology and antimicrobial susceptibility.