ISSN 0886-3067


Volume No 35 Issue No 2

Evaluation of Chitosan; a Thermosensitive Hydrogel Drug Delivery Agent for Loading Dexamethosone

Purpose: To evaluate the characteristics of thermosensitive hydrogel (Chitosan) drug delivery agent loaded with dexamethasone/chitosan which can be used in the treatment of macular edema and non infectious uveitis applied in sub tenon space. Study Design: Quasi experimental study. Place and Duration of Study: Lahore General hospital, IRCBM COMSATS Institute of Information Technology, Lahore, Pakistan from July 2017 to July 2018. Material and Methods: Acetic acid (0.5 M, 2.5 ml) and Chitosan (0.2g) were dissolved and stirred for 1 hour and 30 minutes at room temperature. Powdered dexamethasone (3.5 mg) was added and stirred for further 30 min at room temperature. This solution was placed at 4C for 30 min to cool it down. After this, NaHCO3 solution (0.48 M, 2 ml) was added drop wise. Once required pH was achieved, solution was placed inside oven at 37C. Formation of gel started after 3-5 minutes and it took 2 hours for complete conversion of liquid into hydrogel. Results: The gelling time of the synthesized gel was 2 hours and was tested by test tube invert method. To prove the non-irritancy of thermosensitive hydrogel, Hens Egg Test on Chorioallantoic membrane assay (HET-CAM) was performed. Results showed that synthesized hydrogel was non-irritant. Outcomes of in vitro degradation tests displayed that synthesized hydrogels were biodegradable. The drug release tests revealed that synthesized hydrogels displayed sustained release of drug. Conclusion: The analysis showed that physical changes and drug loading did not alter the chemical structure of chitosan therefore it is an effective potential vehicle for slow release of Dexamethasone if placed in the sub tenon space. Keywords: Chitosan, dexamethasone, macular edema, non-infectious uveitis.

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